Journal: Oncotarget

Article Title: By inhibiting snail signaling and miR-23a-3p, osthole suppresses the EMT-mediated metastatic ability in prostate cancer

doi:

Figure Lengend Snippet: A. A schematic representation of the procedure for miRNA selection. Differential expressions of miRNAs in osthole-treated cells versus vehicle-treated cells were analyzed with a OneArray ® miRNA profiling chip. B. Treatment of Du145 cells with osthole for 6 h. miR-146a, miR-22-3p, and miR-23a-3p expressions were detected by a quantitative PCR. C. Upper panel, Du145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. E-cadherin expression levels were determined by a Western blot analysis. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level. Lower panel, Relative luciferase activities of DU145 cells co-transfected with an E-cadherin luciferase 3′UTR reporter vector and miR-23a-3p mimic or mimic control for 24 h. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. D. DU145 cells were transfected with an miR-23a-3p mimic or mimic control for 24 h followed by osthole (60 μM) treatment for an additional 24 h. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. Data were analyzed using a one-way ANOVA with Tukey's post-hoc tests at 95% confidence intervals; different letters represent different levels of significance. E. DU145 cells were transfected with either an miR-23a-3p inhibitor or a negative control. The cell-invasion ability was determined by a Matrigel invasion assay. Values are presented as the mean ± SE of three independent experiments. * p < 0.05, compared to the control groups. F. PC3 or DU145 cells were treated with TGF-β for 6, 12, or 24 h. Expression levels of E-cadherin and miR-23a-3p were determined by Western blotting (upper panel) and a quantitative PCR (lower panel), respectively. Quantitative E-cadherin protein levels were adjusted to the β-actin protein level.

Article Snippet: To identify which miRNAs are regulated by osthole, a high-throughput and specific miRNA microarray (human miRNA OneArray ® miRNA profiling chip) using Du145 cells after osthole treatment was conducted by the Phalanx Biotech Group (Hsinchu, Taiwan).

Techniques: Selection, Real-time Polymerase Chain Reaction, Transfection, Expressing, Western Blot, Luciferase, Plasmid Preparation, Invasion Assay, Negative Control

Journal: PLoS ONE

Article Title: Reference miRNAs for miRNAome Analysis of Urothelial Carcinomas

doi: 10.1371/journal.pone.0039309

Figure Lengend Snippet: Candidate reference miRNAs selected from microarray analysis. †

Article Snippet: Based on the total of 723 human miRNA species located on the Agilent microarray chip according to the miRBase version 10.1, we identified 101 miRNAs that were flagged as “present” in all of the examined groups ( ).

Techniques: Microarray

Journal: PLoS ONE

Article Title: Molecular Predictors of Long-Term Survival in Glioblastoma Multiforme Patients

doi: 10.1371/journal.pone.0154313

Figure Lengend Snippet: Summary of the 6 types of molecular data and their platforms used for this study.

Article Snippet: miRNA , Agilent 8X15 k human miRNA-specific microarray , 437 , 43/394 , 534 , 1 , 1.

Techniques: Mutagenesis, Genome Wide, DNA Sequencing, Expressing, DNA Methylation Assay, Microarray